Journal: Analytica chimica acta

Article Title: Sorting by Interfacial Tension (SIFT): Label-Free Enzyme Sorting Using Droplet Microfluidics

doi: 10.1016/j.aca.2019.08.025

Figure Lengend Snippet: a) Interfacial tension as of function of pH for droplets of phosphate buffered saline in QX100 Droplet Generation Oil diluted 100 fold by volume with Novec 7500. b) Schematic of droplet sorter using a tapered rail oriented at 45° in relation to the direction of flow. Low pH droplets are indicated in purple while high pH droplets indicated in green. Droplets enter from bottom left channel. Droplets of high pH (low interfacial tension) are only slightly deflected by the rail. However, droplets of low pH (high interfacial tension) enter the rail and follow the rail upwards. The top left inlet, the Oil Entrainment Inlet, allows an independent control of the hydrodynamic drag in the sorting region of the device. The change in shape of the droplets in the rail is exaggerated for illustrative purposes.

Article Snippet: 28 , 29 It was observed that for aqueous droplets in the external oil, QX100 Droplet Generation Oil (Biorad) a product designed for use with the QX200/QX100 droplet digital PCR system, the physical properties of droplets are very sensitive to pH.

Techniques: Saline, Control

Journal: Cell reports

Article Title: Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21

doi: 10.1016/j.celrep.2019.08.036

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DG8 Gaskets for QX200/QX100 Droplet Generator , Bio-Rad , Cat# 1863009.

Techniques: Virus, Recombinant, Modification, Transfection, Protease Inhibitor, Saline, Electron Microscopy, Red Blood Cell Lysis, Plasmid Preparation, Sequencing, DNA Library Preparation, Control, cDNA Synthesis, Bicinchoninic Acid Protein Assay, RNA Sequencing, Expressing, Negative Control, Software, Imaging, Flow Cytometry